Please use this identifier to cite or link to this item: https://doi.org/10.1039/c0mb00327a
Title: Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag
Authors: Lee, J.-J.
Son, J.
Ha, H.-H. 
Chang, Y.-T. 
Issue Date: 1-Apr-2011
Citation: Lee, J.-J., Son, J., Ha, H.-H., Chang, Y.-T. (2011-04-01). Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag. Molecular BioSystems 7 (4) : 1270-1276. ScholarBank@NUS Repository. https://doi.org/10.1039/c0mb00327a
Abstract: Imaging a specific protein of interest in live cell has versatile applications in biological research. Recently, diverse chemical tags have been developed to overcome the limits of autofluorescence protein (FP) tags. However, current chemical methods still need to be progressed to compete with FPs in the scope of specificity and convenience in staining procedure. We report a novel protein tagging method that provides a convenient and specific fluorescent labeling of membrane proteins. Ω tag is developed by employing a mammalian enzyme glutathione sulfur-transferase omega 1 (GSTO1) and its partner dye, 5-bromomethyl fluorescein (BMF). Ω-tagged membrane proteins were labeled by BMF efficiently for live cell imaging and in-gel analysis. Endocytosis of epidermal growth factor receptor (EGFR) was successfully visualized by using this Ω tagging system. Ω tag will provide a convenient tool for the physiological study of membrane proteins in live cells. © The Royal Society of Chemistry.
Source Title: Molecular BioSystems
URI: http://scholarbank.nus.edu.sg/handle/10635/76196
ISSN: 1742206X
DOI: 10.1039/c0mb00327a
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