Please use this identifier to cite or link to this item: https://doi.org/10.1039/c3cc42836b
Title: Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)
Authors: Kalidasan, K.
Su, Y.
Wu, X.
Yao, S.Q. 
Uttamchandani, M. 
Issue Date: 21-Aug-2013
Source: Kalidasan, K., Su, Y., Wu, X., Yao, S.Q., Uttamchandani, M. (2013-08-21). Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP). Chemical Communications 49 (65) : 7237-7239. ScholarBank@NUS Repository. https://doi.org/10.1039/c3cc42836b
Abstract: An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity. © 2013 The Royal Society of Chemistry.
Source Title: Chemical Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/76192
ISSN: 13597345
DOI: 10.1039/c3cc42836b
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