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https://doi.org/10.1039/c3cc42836b
| Title: | Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP) |
| Authors: | Kalidasan, K. Su, Y. Wu, X. Yao, S.Q. Uttamchandani, M. |
| Issue Date: | 21-Aug-2013 |
| Citation: | Kalidasan, K., Su, Y., Wu, X., Yao, S.Q., Uttamchandani, M. (2013-08-21). Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP). Chemical Communications 49 (65) : 7237-7239. ScholarBank@NUS Repository. https://doi.org/10.1039/c3cc42836b |
| Abstract: | An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity. © 2013 The Royal Society of Chemistry. |
| Source Title: | Chemical Communications |
| URI: | http://scholarbank.nus.edu.sg/handle/10635/76192 |
| ISSN: | 13597345 |
| DOI: | 10.1039/c3cc42836b |
| Appears in Collections: | Staff Publications |
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