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|Title:||Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)|
|Citation:||Kalidasan, K., Su, Y., Wu, X., Yao, S.Q., Uttamchandani, M. (2013-08-21). Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP). Chemical Communications 49 (65) : 7237-7239. ScholarBank@NUS Repository. https://doi.org/10.1039/c3cc42836b|
|Abstract:||An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity. © 2013 The Royal Society of Chemistry.|
|Source Title:||Chemical Communications|
|Appears in Collections:||Staff Publications|
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