Enantiomeric separation of amino acids derivatized with fluorescence isothiocyanate isomer I by micellar electrokinetic chromatography using β- and γ-cyclodextrins as chiral selectors

Abstract

Enantiomeric separation of 21 amino acids derivatized with fluoresceine isothiocyanate isomer I (FITC) has been studied by micellar electrokinetic chromatography using β-and γ-cyclodextrin (CD) as chiral selectors. Chiral resolution of 21 FITC derivatives of amino acids was achieved with both β- and γ-CD in 100 mM borate buffer (pH 9.5) containing 30 mM sodium dodecyl sulfate (SDS). The effects of CD concentration, SDS concentration and organic modifiers concentration as well as capillary length were investigated. Chiral recognition capability of β- and γ-CD was compared. γ-CD was found to be a better chiral selector than β-CD in terms of chiral resolution capability for FITC-amino acids.