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https://doi.org/10.1016/j.jdent.2007.07.012
Title: | Enamel diffusion modulated by Er:YAG laser. (Part 2). Organic matrix | Authors: | Maung, N.L. Wohland, T. Hsu, C.-Y.S. |
Keywords: | CLSM Diffusion Enamel Er:YAG laser Organic matrix |
Issue Date: | Oct-2007 | Citation: | Maung, N.L., Wohland, T., Hsu, C.-Y.S. (2007-10). Enamel diffusion modulated by Er:YAG laser. (Part 2). Organic matrix. Journal of Dentistry 35 (10) : 794-799. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jdent.2007.07.012 | Abstract: | Organic matrix (OM) has been hypothesized as a key player in the laser-induced retardation of enamel diffusion (LRED). Objectives: Therefore, this study was aimed to quantify the contribution of OM in LRED. Methods: Four groups of enamel sections (n = 10) were assigned to 'normal', 'laser treated', 'OM extracted' and 'laser + OM extraction' groups for measurement of diffusion coefficient (DC) using fluorescence recovery after photobleaching (FRAP) and fluorophores transport study (FTS). Er:YAG laser treatment and OM extraction were performed on respective groups. Sections were characterized with stereomicroscopy and polarized light microscopy. Treatment effects were statistically assessed with a factorial ANOVA. Results: DC measured by FRAP and FTS coupled with confocal microscopy revealed the significant effect of OM (p = 0.001) and laser treatment (p < 0.01). After OM extraction, the laser effect on diffusion decreased about 34-75%, confirming the significant role of OM in LRED. Conclusion: Both FRAP and FTS may be promising tools to quantify enamel DC. © 2007. | Source Title: | Journal of Dentistry | URI: | http://scholarbank.nus.edu.sg/handle/10635/76091 | ISSN: | 03005712 | DOI: | 10.1016/j.jdent.2007.07.012 |
Appears in Collections: | Staff Publications |
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