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Title: Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis
Authors: Zhang, W.
Popovich, D.G. 
Keywords: Apoptosis
Issue Date: 23-Apr-2008
Citation: Zhang, W., Popovich, D.G. (2008-04-23). Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis. Journal of Agricultural and Food Chemistry 56 (8) : 2603-2608. ScholarBank@NUS Repository.
Abstract: The growth inhibition and the induction of apoptosis brought about by soyasaponins extracted from soy flour (Glycine max (L.)) and concentrated for soyasapogenols A and B formed by hydrolysis were tested for cytoactivity in the human hepatocellular carcinoma cell line Hep-G2. Concentrated soyasapogenol A (SG-A) and soyasapogenol B (SG-B) extracts contained approximately 69.3% and 46.2% of their respective aglycones (soyasapogenols) assessed by HPLC and ESI-MS, while the soyasaponin extract (TS), derived from crude methanol extraction, did not contain any detectable amounts of SG-A or SG-B. An MTT viability assay showed that all three extracts had an effect on Hep-G2 proliferation in a dose-response manner with 72 h LC50 values of 0.594 ± 0.021 mg/mL for TS, 0.052 ± 0.011 mg/mL for SG-A, and 0.128 ± 0.005 mg/mL for SG-B. Apoptotic cells were determined by flow cytometry cell cycle analysis and confocal laser scanning microscopy (CLSM). Cell cycle analysis indicated a significant (P < 0.05) greater sub-G1 buildup of apoptotic cells at 24 h (25.63 ± 2.1%) and 72 h (47.1 ± 3.5%) for the SG-A extract compared to SG-B, whereas the TS extract produced only a minor buildup of sub-G1 cells. CLSM confirmed a morphological change of all treatments after 24 h, at the respective LC50 concentrations. These results show that the samples that contained mainly soyasapogenols A and B showed a greater ability to inhibit proliferation of cultured Hep-G2 when compared to a total soyasaponin extract that did not contain any soyasapogenols. © 2008 American Chemical Society.
Source Title: Journal of Agricultural and Food Chemistry
ISSN: 00218561
DOI: 10.1021/jf0731550
Appears in Collections:Staff Publications

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