Please use this identifier to cite or link to this item:
|Title:||Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis|
|Citation:||Zhang, W., Popovich, D.G. (2008-04-23). Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis. Journal of Agricultural and Food Chemistry 56 (8) : 2603-2608. ScholarBank@NUS Repository. https://doi.org/10.1021/jf0731550|
|Abstract:||The growth inhibition and the induction of apoptosis brought about by soyasaponins extracted from soy flour (Glycine max (L.)) and concentrated for soyasapogenols A and B formed by hydrolysis were tested for cytoactivity in the human hepatocellular carcinoma cell line Hep-G2. Concentrated soyasapogenol A (SG-A) and soyasapogenol B (SG-B) extracts contained approximately 69.3% and 46.2% of their respective aglycones (soyasapogenols) assessed by HPLC and ESI-MS, while the soyasaponin extract (TS), derived from crude methanol extraction, did not contain any detectable amounts of SG-A or SG-B. An MTT viability assay showed that all three extracts had an effect on Hep-G2 proliferation in a dose-response manner with 72 h LC50 values of 0.594 ± 0.021 mg/mL for TS, 0.052 ± 0.011 mg/mL for SG-A, and 0.128 ± 0.005 mg/mL for SG-B. Apoptotic cells were determined by flow cytometry cell cycle analysis and confocal laser scanning microscopy (CLSM). Cell cycle analysis indicated a significant (P < 0.05) greater sub-G1 buildup of apoptotic cells at 24 h (25.63 ± 2.1%) and 72 h (47.1 ± 3.5%) for the SG-A extract compared to SG-B, whereas the TS extract produced only a minor buildup of sub-G1 cells. CLSM confirmed a morphological change of all treatments after 24 h, at the respective LC50 concentrations. These results show that the samples that contained mainly soyasapogenols A and B showed a greater ability to inhibit proliferation of cultured Hep-G2 when compared to a total soyasaponin extract that did not contain any soyasapogenols. © 2008 American Chemical Society.|
|Source Title:||Journal of Agricultural and Food Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on Apr 24, 2018
checked on Apr 20, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.