Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/76023
Title: Effect of NaOH on large-volume sample stacking of haloacetic acids in capillary zone electrophoresis with a low-pH buffer
Authors: Tu, C.
Zhu, L.
Ang, C.H.
Lee, H.K. 
Keywords: Capillary zone electrophoresis
Haloacetic acids
Large-volume sample stacking
Issue Date: Jun-2003
Source: Tu, C.,Zhu, L.,Ang, C.H.,Lee, H.K. (2003-06). Effect of NaOH on large-volume sample stacking of haloacetic acids in capillary zone electrophoresis with a low-pH buffer. Electrophoresis 24 (12-13) : 2188-2192. ScholarBank@NUS Repository.
Abstract: Large-volume sample stacking (LVSS) is an effective on-capillary sample concentration method in capillary zone electrophoresis, which can be applied to the sample in a low-conductivity matrix. NaOH solution is commonly used to back-extract acidic compounds from organic solvent in sample pretreatment. The effect of NaOH as sample matrix on LVSS of haloacetic acids was investigated in this study. It was found that the presence of NaOH in sample did not compromise, but rather help the sample stacking performance if a low pH background electrolyte (BGE) was used. The sensitivity enhancement factor was higher than the case when sample was dissolved in pure water or diluted BGE. Compared with conventional injection (0.4% capillary volume), 97-120-fold sensitivity enhancement in terms of peak height was obtained without deterioration of separation with an injection amount equal to 20% of the capillary volume. This method was applied to determine haloacetic acids in tap water by combination with liquid-liquid extraction and back-extraction into NaOH solution. Limits of detection at sub-ppb levels were obtained for real samples with direct UV detection.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/76023
ISSN: 01730835
Appears in Collections:Staff Publications

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