Please use this identifier to cite or link to this item: https://doi.org/10.1002/cphc.200301057
Title: Dual-color fluorescence cross-correlation spectroscopy using single laser wavelength excitation
Authors: Hwang, L.C.
Wohland, T. 
Keywords: FRET (fluorescence resonance energy transfer)
Ligands
Quantum dots
Receptors
Spectroscopic methods
Issue Date: 19-Apr-2004
Citation: Hwang, L.C., Wohland, T. (2004-04-19). Dual-color fluorescence cross-correlation spectroscopy using single laser wavelength excitation. ChemPhysChem 5 (4) : 549-551. ScholarBank@NUS Repository. https://doi.org/10.1002/cphc.200301057
Abstract: Spotlight on interactions: The authors show that single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS) is possible using only one laser beam to excite a combination of labels, in work performed with studied FRET (fluorescence resonance energy transfer) dyes and quantum dots. They demonstrate that equilibrium binding and stoichiometry can easily be determined (see graphic; QR = quantum red dye, BF = fluorescein-labeled biotin). SW-FCCS is a promising tool for high-throughput screening applications as well as for the measurement of molecular interactions.
Source Title: ChemPhysChem
URI: http://scholarbank.nus.edu.sg/handle/10635/75989
ISSN: 14394235
DOI: 10.1002/cphc.200301057
Appears in Collections:Staff Publications

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