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|Title:||Developing site-specific immobilization strategies of peptides in a microarray|
|Authors:||Lesaicherre, M.-L. |
|Source:||Lesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Developing site-specific immobilization strategies of peptides in a microarray. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2079-2083. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(02)00379-7|
|Abstract:||In peptide-based microarrays, most existing methods do not allow for site-specific immobilization of peptides on the glass surface. We have developed two new approaches for site-specific immobilization of kinase substrates onto glass slides: (1) slides were functionalized with avidin for attachment of biotinylated peptides; and (2) slides were functionalized with thioester for attachment of N-terminally cysteine-containing peptides via a native chemical ligation reaction. © 2002 Elsevier Science Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
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