Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200500819
Title: Determination of tobramycin in human serum by capillary electrophoresis with contactless conductivity detection
Authors: Law, W.S. 
Kubáň, P.
Yuan, L.L.
Zhao, J.H.
Li, S.F.Y. 
Hauser, P.C.
Keywords: Capacitively coupled contactless conductivity detection
Capillary electrophoresis
Field-amplified sample stacking
Tobramycin
Issue Date: May-2006
Source: Law, W.S., Kubáň, P., Yuan, L.L., Zhao, J.H., Li, S.F.Y., Hauser, P.C. (2006-05). Determination of tobramycin in human serum by capillary electrophoresis with contactless conductivity detection. Electrophoresis 27 (10) : 1932-1938. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200500819
Abstract: A study on the determination of the antibiotic tobramycin by CE with capacitively coupled contactless conductivity detection is presented. This method enabled the direct quantification of the non-UV-absorbing species without incurring the disadvantages of the indirect approaches which would be needed for optical detection. The separation of tobramycin from inorganic cations present in serum samples was achieved by optimizing the composition of the acetic acid buffer. Field-amplified sample stacking was employed to enhance the sensitivity of the method and a detection limit of 50 μg/L (S/N = 3) was reached. The RSDs obtained for migration time and peak area using kanamycin B as internal standard were typically 0.12 and 4%, respectively. The newly developed method was validated by measuring the concentration of tobramycin in serum standards containing typical therapeutic concentrations of 2 and 10 mg/L. The recoveries were 96 and 97% for the two concentrations, respectively. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/75907
ISSN: 01730835
DOI: 10.1002/elps.200500819
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