Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/75891
Title: Determination of erythrocyte porphyrins by EPI-illumination fluorescence microscope with capillary electrophoresis
Authors: Yao, Y.J.
Li, S.F.Y. 
Issue Date: 1996
Citation: Yao, Y.J.,Li, S.F.Y. (1996). Determination of erythrocyte porphyrins by EPI-illumination fluorescence microscope with capillary electrophoresis. Journal of Liquid Chromatography and Related Technologies 19 (1) : 1-15. ScholarBank@NUS Repository.
Abstract: A micellar electrokinetic capillary chromatographic (MEKC) method is described for the simultaneous determination of zinc protoporphyrin (ZnPP) and protoporphyrin (PP) in whole blood. Reproducibility of migration times and peak areas of ZnPP and PP have been shown to improve significantly with the use of an internal standard. Erythrocyte porphyrins were extracted from whole blood with N,N-dimethylformamide (DMF), followed by sample cleanup and preconcentration on C-18 cartridges. Analytical recoveries of the porphyrins were better than 88%. An epi-illumination fluorescence microscope was used for the detection of these porphyrins. Limits of detection were found to be 50 nM and 8 nM for ZnPP and PP, respectively.
Source Title: Journal of Liquid Chromatography and Related Technologies
URI: http://scholarbank.nus.edu.sg/handle/10635/75891
ISSN: 10826076
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