Please use this identifier to cite or link to this item: https://doi.org/10.1016/S1389-1723(02)80070-6
Title: Complete cDNA sequence of chitin deacetylase from Gongronella butleri and its phylogenetic analysis revealed clusters corresponding to taxonomic classification of fungi
Authors: Maw, T.
Tan, T.K. 
Khor, E. 
Wong, S.M. 
Keywords: cDNA
Chitin deacetylase
Gongronella butleri
Taxonomic classification
Issue Date: 2002
Source: Maw, T.,Tan, T.K.,Khor, E.,Wong, S.M. (2002). Complete cDNA sequence of chitin deacetylase from Gongronella butleri and its phylogenetic analysis revealed clusters corresponding to taxonomic classification of fungi. Journal of Bioscience and Bioengineering 93 (4) : 376-381. ScholarBank@NUS Repository. https://doi.org/10.1016/S1389-1723(02)80070-6
Abstract: A cDNA library containing a chitin deacetylase (CDA) gene from a zygomycete Gongronella butleri was constructed and the complete gene was sequenced. The complete gene contains an open reading frame of 1290 nucleotides which encodes a sequence of 430 amino acid residues. The gene sequence consists of nucleotides encoding a polysaccharide deacetylase domain located in the middle, covering 34% of the entire sequence. This domain shares high sequence similarities with CDAs of fungi and other deacetylases including the nodb-like protein from Sinorhizobium meliloti and Bacillus subtilis, xylanase D from Cellulomonas fimi, and acetylxylan esterase A from Streptomyces lividans. The deduced amino acid sequence shows homology with CDAs from Mucor rouxii (48% identity) and Phycomyces blakesleeanus (31)% identity). Phylogenetic analysis reveals that CDAs are clustered in accordance with the taxonomic classification of the fungi.
Source Title: Journal of Bioscience and Bioengineering
URI: http://scholarbank.nus.edu.sg/handle/10635/75789
ISSN: 13891723
DOI: 10.1016/S1389-1723(02)80070-6
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