Please use this identifier to cite or link to this item:
Title: Comparison of Supercritical Fluid Chromatographic and High-Performance Liquid Chromatographic Separations of p-tert-Butylcalix[n]arenes
Authors: Graham, B.F.
Harrowfield, J.M.
Trengove, R.D.
Rodriguez, I.
Li, S.F.Y. 
Issue Date: May-1997
Source: Graham, B.F.,Harrowfield, J.M.,Trengove, R.D.,Rodriguez, I.,Li, S.F.Y. (1997-05). Comparison of Supercritical Fluid Chromatographic and High-Performance Liquid Chromatographic Separations of p-tert-Butylcalix[n]arenes. Journal of Chromatographic Science 35 (5) : 232-236. ScholarBank@NUS Repository.
Abstract: Supercritical fluid chromatography (SFC) using packed analytical columns is compared with high-performance liquid chromatography (HPLC) for the separation of the readily available calixarenes, p-tert-butylcalix[4]arene, p-tert-butylcalix[6]arene, and p-tert-butylcalix[8]arene. With methanol as a modifier in supercritical carbon dioxide, p-tert-butylcalix[8]arene fails to elute from any of the four different columns used (ODS, RP-18, Cyano, and Diol), although the smaller calixarenes elute; p-tert-butylcalix[4]arene always precedes p-tert-butylcalix[6]arene. By using chloroform modifier under gradient conditions, all three can be resolved, and a retention time as short as 1.4 min is obtained for p-tert-butylcalix[8]arene on an RP-18 column. The total run time including re-equilibration is slightly over 3 min. In contrast, the shortest retention time obtained for p-tert-butylcalix[8]arene by HPLC (150-mm ODS column, 3-μm particle size, isocratic conditions, acetonitrile-ethyl acetate mobile phase [80:20]) is approximately 5 min. Peaks are both narrower and more symmetrical under SFC rather than HPLC conditions.
Source Title: Journal of Chromatographic Science
ISSN: 00219665
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Mar 9, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.