Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/75784
Title: Comparison of Supercritical Fluid Chromatographic and High-Performance Liquid Chromatographic Separations of p-tert-Butylcalix[n]arenes
Authors: Graham, B.F.
Harrowfield, J.M.
Trengove, R.D.
Rodriguez, I.
Li, S.F.Y. 
Issue Date: May-1997
Source: Graham, B.F.,Harrowfield, J.M.,Trengove, R.D.,Rodriguez, I.,Li, S.F.Y. (1997-05). Comparison of Supercritical Fluid Chromatographic and High-Performance Liquid Chromatographic Separations of p-tert-Butylcalix[n]arenes. Journal of Chromatographic Science 35 (5) : 232-236. ScholarBank@NUS Repository.
Abstract: Supercritical fluid chromatography (SFC) using packed analytical columns is compared with high-performance liquid chromatography (HPLC) for the separation of the readily available calixarenes, p-tert-butylcalix[4]arene, p-tert-butylcalix[6]arene, and p-tert-butylcalix[8]arene. With methanol as a modifier in supercritical carbon dioxide, p-tert-butylcalix[8]arene fails to elute from any of the four different columns used (ODS, RP-18, Cyano, and Diol), although the smaller calixarenes elute; p-tert-butylcalix[4]arene always precedes p-tert-butylcalix[6]arene. By using chloroform modifier under gradient conditions, all three can be resolved, and a retention time as short as 1.4 min is obtained for p-tert-butylcalix[8]arene on an RP-18 column. The total run time including re-equilibration is slightly over 3 min. In contrast, the shortest retention time obtained for p-tert-butylcalix[8]arene by HPLC (150-mm ODS column, 3-μm particle size, isocratic conditions, acetonitrile-ethyl acetate mobile phase [80:20]) is approximately 5 min. Peaks are both narrower and more symmetrical under SFC rather than HPLC conditions.
Source Title: Journal of Chromatographic Science
URI: http://scholarbank.nus.edu.sg/handle/10635/75784
ISSN: 00219665
Appears in Collections:Staff Publications

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