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https://doi.org/10.1116/1.3474475
DC Field | Value | |
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dc.title | cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor | |
dc.contributor.author | Ng, J.F. | |
dc.contributor.author | Jaenicke, S. | |
dc.contributor.author | Eisele, K. | |
dc.contributor.author | Dorn, J. | |
dc.contributor.author | Weil, T. | |
dc.date.accessioned | 2014-06-23T05:33:39Z | |
dc.date.available | 2014-06-23T05:33:39Z | |
dc.date.issued | 2010-09 | |
dc.identifier.citation | Ng, J.F., Jaenicke, S., Eisele, K., Dorn, J., Weil, T. (2010-09). cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor. Biointerphases 5 (3) : FA41-FA47. ScholarBank@NUS Repository. https://doi.org/10.1116/1.3474475 | |
dc.identifier.issn | 19348630 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/75706 | |
dc.description.abstract | Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin (cBSA-147) has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column (inside diameter of 530 μm and L=30 m) and evaluated in the enantioselective reduction of ethyl acetoacetate to R-(-)ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization. © 2010 American Vacuum Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1116/1.3474475 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | CHEMISTRY | |
dc.description.doi | 10.1116/1.3474475 | |
dc.description.sourcetitle | Biointerphases | |
dc.description.volume | 5 | |
dc.description.issue | 3 | |
dc.description.page | FA41-FA47 | |
dc.identifier.isiut | 000285473600008 | |
Appears in Collections: | Staff Publications |
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