Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(97)01149-7
Title: Capillary electrophoretic analysis of cyclodextrins with dynamic fluorescence labeling and detection
Authors: Wei, H.
Li, S.F.Y. 
Keywords: Aminonaphthalenesulfonic acid
Complexation
Cyclodextrins
Issue Date: 27-Mar-1998
Citation: Wei, H., Li, S.F.Y. (1998-03-27). Capillary electrophoretic analysis of cyclodextrins with dynamic fluorescence labeling and detection. Journal of Chromatography A 800 (2) : 333-338. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(97)01149-7
Abstract: The use of 8-anilinonaphthalene-1-sulfonic acid (8,1-ANS) as buffer additive in the capillary electrophoretic separation of cyclodextrins (CDs) was investigated. Better detection sensitivity was obtained for α- and γ- CDs than with previously reported capillary electrophoretic methods. Increasing the concentration of 8, 1-ANS improved resolution and sensitivities for α-, β- and γ-CDs, while decreasing the pH of the background electrolyte can improve sensitivities. Detection limits for α-, β- and γ-CDs were determined to be 60, 20 and 7 μM, respectively. The formation constants of CD-8,1-ANS complexes at pH 6 were also measured by capillary electrophoresis. Finally the specificity of amyloglucosidase to CDs was analyzed as a practical application of this method.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/75689
ISSN: 00219673
DOI: 10.1016/S0021-9673(97)01149-7
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Dec 12, 2018

WEB OF SCIENCETM
Citations

8
checked on Dec 12, 2018

Page view(s)

30
checked on Dec 8, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.