Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2013.04.009
Title: An aptamer based surface plasmon resonance biosensor for the detection of bovine catalase in milk
Authors: Ashley, J.
Li, S.F.Y. 
Keywords: Aptamer
Bovine catalase
Milk
SPR
Surface plasmon resonance
Issue Date: 5-Oct-2013
Citation: Ashley, J., Li, S.F.Y. (2013-10-05). An aptamer based surface plasmon resonance biosensor for the detection of bovine catalase in milk. Biosensors and Bioelectronics 48 : 126-131. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2013.04.009
Abstract: In this research, we report the development of an aptamer based SPR biosensor for the detection of catalase in milk samples with minimal sample preparation. A biotin tagged aptamer was immobilized onto a gold surface by affinity capture. A limit of detection (LOD) in the nanomolar range (20.5. nM, RSD: 15.2%) was found and a dynamic range of 15-1000. nM was established for catalase in buffer and the aptamer showed good specificity toward catalase. This biosensor has the potential to be used in the detection of catalase in milk samples, a key indicator of mastitis disease in milk. © 2013 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/75556
ISSN: 09565663
DOI: 10.1016/j.bios.2013.04.009
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