Please use this identifier to cite or link to this item: https://doi.org/10.1242/jcs.051557
Title: Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative and recycling compartments
Authors: Zhang, D.
Manna, M.
Wohland, T. 
Kraut, R.
Keywords: FCS
Microdomain
Raft
SBD
Sphingolipid
Trafficking
Issue Date: 15-Oct-2009
Citation: Zhang, D., Manna, M., Wohland, T., Kraut, R. (2009-10-15). Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative and recycling compartments. Journal of Cell Science 122 (20) : 3715-3728. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.051557
Abstract: Several cholesterol-dependent cellular uptake pathways involving microdomain-resident sphingolipids have been characterized, but little is known about what controls the further intracellular trafficking routes of those domains. Here, we present evidence that the uptake and intracellular trafficking of a recently described sphingolipid-binding probe, the sphingolipid binding domain (SBD) peptide, is mediated by two parallel cooperating mechanisms requiring flotillin, dynamin and cdc42, which act in concert to direct a distinct surface behavior and trafficking itinerary. Diffusion measurements of SBD at the cell surface by fluorescence correlation spectroscopy suggest that cdc42- and flotillin-associated uptake sites both correspond to domains of intermediate mobility, but that they can cooperate to form low-mobility, efficiently internalized domains. Interestingly, we find that the choice of uptake mechanism affects subsequent trafficking of SBD, as does cholesterol content. Interference with one or other uptake pathway acts as a toggle switch for the trafficking of SBD to recycling endosomes or endolysosomes, whereas both of these pathways are bypassed if cholesterol is reduced. The data are in accordance with a scenario in which SBD mirrors the trafficking response of raft-borne lipids towards a degradative or recycling target. In summary, we suggest that both the surface behavior of a cargo and its subsequent trafficking are determined by a combination of endocytic accessory proteins and the cholesterol content of different membrane compartments.
Source Title: Journal of Cell Science
URI: http://scholarbank.nus.edu.sg/handle/10635/75544
ISSN: 00219533
DOI: 10.1242/jcs.051557
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on May 21, 2018

WEB OF SCIENCETM
Citations

11
checked on May 1, 2018

Page view(s)

45
checked on May 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.