Please use this identifier to cite or link to this item:
|Title:||A highly sensitive and selective homogenous assay for profiling microRNA expression|
|Citation:||Deng, H., Shen, W., Ren, Y., Gao, Z. (2014-04-15). A highly sensitive and selective homogenous assay for profiling microRNA expression. Biosensors and Bioelectronics 54 : 650-655. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2013.11.046|
|Abstract:||A highly sensitive and selective homogeneous microRNA (miRNA) assay is described in this report. It is based on the cleavage of DNAzyme moieties from miRNA-hybridized DNAzyme-capped capture probes (DZ-CPs) from magnetic beads by a duplex-specific nuclease (DSN). After removing the magnetic beads together with the unreacted DZ-CPs by using a permanent magnet, amplified colorimetric detection of the target miRNA is realized through the cleaved DNAzyme moieties-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine. The exceptional amplification power of the DSN and the cumulative nature of the signal generation process permit the colorimetric detection of miRNAs down to subfemtomolar levels. The isothermal amplification scheme together with the simple assay protocol makes direct profiling miRNA in real-world samples possible. © 2013 Elsevier B.V.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 14, 2018
WEB OF SCIENCETM
checked on Jun 19, 2018
checked on Jun 29, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.