Please use this identifier to cite or link to this item: https://doi.org/10.1016/0021-9673(95)92842-C
Title: Preparative-scale liquid chromatographic separation of ω-3 fatty acids from fish oil sources
Authors: Hidajat, K. 
Ching, C.B. 
Rao, M.S.
Issue Date: 1995
Citation: Hidajat, K., Ching, C.B., Rao, M.S. (1995). Preparative-scale liquid chromatographic separation of ω-3 fatty acids from fish oil sources. Journal of Chromatography A 702 (1-2) : 215-221. ScholarBank@NUS Repository. https://doi.org/10.1016/0021-9673(95)92842-C
Abstract: A preparative-scale chromatographic process for the enrichment and isolation of closely related compounds such as ω-3 fatty acids from fish oil supplements is described. An analytical-scale procedure was scaled up to a preparative level process to provide a simple, rapid, selective and sensitive method for the fractionation of these essential acids. The fish oil triglycerides were saponified into their free fatty acids. The polyunsaturated fatty acids were then enriched through urea crystallization. Subsequently, the individual ω-3 fatty acids were fractionated on a Waters PrepPak unit consisting of six cartridges (μBondapak phenyl silica) using an isocratic ternary mobile phase of acetonitrile-water-tetrahydrofuran (45:35:20, v/v/v). The separation was accomplished within 60 min without any recycling of the solutes. The fractions collected were further analysed by analytical liquid chromatography.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/75307
ISSN: 00219673
DOI: 10.1016/0021-9673(95)92842-C
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

5
checked on Jul 13, 2018

WEB OF SCIENCETM
Citations

5
checked on Jun 11, 2018

Page view(s)

65
checked on Jul 6, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.