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|Title:||Culture development from a DNAPL zone for high 1,1,1-TCA and TCE bioremediation|
|Citation:||Pon, G.,Fam, S.A.,Mountain, S.,Falatko, D.,He, J.,Chang, D. (2009). Culture development from a DNAPL zone for high 1,1,1-TCA and TCE bioremediation. In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium. ScholarBank@NUS Repository.|
|Abstract:||Bioremediation of a mixture of 1,1,1-trichloroethane (TCA) and trichloroethene (TCE) is significantly more challenging than bioremediation of tetrachloroethene (PCE) and trichloroethene (TCE) series compounds. We developed a mixed culture originating from a high concentration TCA source zone. In its natural environment, the culture also tolerated very high concentrations of TCE, cis 1,2 -DCE (hundreds of ppm). This culture degrades TCA, through 1,1-dichloroethane (DCA) to chloroethane (CA). The culture can also degrade high concentration of PCE and TCE. This culture can degrade TCA concentrations up to 600 ppm TCA (with difficulty), but is routinely grown on 200 ppm of TCA. The culture can also degrade low levels of chloroform through dichloromethane to chloromethane. Our historical experience had indicated that TCA biodegradation is concentration sensitive with inhibition starting as low as 35-70 mg/L of TCA without biouagmentation of this NJ-14 variant culture (called NJ-14-5). This study has expanded the inhibition limitation to 200 ppm. We have seen this limit extended at two of our project sites where TCA concentrations are in the 200 ppm range. Detailed inhibition studies on TCE and c-DCE will also be discussed as will be preliminary genetic testing results. A practical attempt to grow this culture on TCE NAPL to enhance TCE NAPL dissolution is also in progress.|
|Source Title:||In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium|
|Appears in Collections:||Staff Publications|
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