Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.904115
Title: Line-scan Focal Modulation Microscopy for rapid imaging of thick biological specimens
Authors: Chong, S.P.
Pant, S.
Chen, N. 
Issue Date: 2011
Citation: Chong, S.P., Pant, S., Chen, N. (2011). Line-scan Focal Modulation Microscopy for rapid imaging of thick biological specimens. Proceedings of SPIE - The International Society for Optical Engineering 8311 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.904115
Abstract: In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal Modulation Microscopy [1- 3] (FMM) is an emerging single-photon excitation fluorescence microscopy technique that can provide sub-micron spatial resolution for deep imaging of biological tissues mainly by preserving the signal-to-background ratio. Here we report a linescan FMM that enables line-by-line recording [4] at video frame rates (>30 fps) depending on the size of region of interest. © 2011 SPIE-OSA-IEEE.
Source Title: Proceedings of SPIE - The International Society for Optical Engineering
URI: http://scholarbank.nus.edu.sg/handle/10635/74889
ISBN: 9780819489593
ISSN: 0277786X
DOI: 10.1117/12.904115
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