Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.908200
Title: An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis
Authors: Lin, J.
Lu, F. 
Zheng, W. 
Yu, H. 
Sheppard, C. 
Huang, Z. 
Keywords: coherent anti-Stokes Raman scattering
collagen
fatty liver
fibrosis
hepatic fibrosis
multimodal nonlinear optical microscopy
second harmonic generation
two photon excitation fluorescence
Issue Date: 2012
Source: Lin, J., Lu, F., Zheng, W., Yu, H., Sheppard, C., Huang, Z. (2012). An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8226 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.908200
Abstract: Liver steatosis and fibrosis are two prevalence liver diseases and may eventually develop into hepatocellular carcinoma (HCC) Due to their prevalence and severity, much work has been done to develop efficient diagnostic methods and therapies. Nonlinear optical microscopy has high sensitivity and chemical specificity for major biochemical compounds, making it a powerful tool for tissue imaging without staining. In this study, three nonlinear microscopy imaging modalities are applied to the study of liver diseases in a bile duct ligation rat modal. CARS shows the distributions of fats or lipids quantitatively across the tissue; SHG visualizes the collagens; and TPEF reveals the morphology of hepatic cells. The results clearly show the development of liver steatosis and fibrosis with time, and the hepatic fat and collagen fibrils are quantified. This study demonstrates the ability of multimodal nonlinear optical microscopy for liver disease diagnosis, and may provide new insights into the understanding of the mechanisms of steatosis/fibrosis transformations at the cellular and molecular levels. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
URI: http://scholarbank.nus.edu.sg/handle/10635/74823
ISBN: 9780819488695
ISSN: 16057422
DOI: 10.1117/12.908200
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