Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/74620
Title: Immobilization of enzymes on functionalized magnetic nanoparticles for efficient biocatalysis
Authors: Wang, W.
Li, Z. 
Wang, D.I.C.
Keywords: Biocatalysis
BSA
Immobilization of enzymes
Magnetic nanoparticles
Superparamagnetism
Issue Date: 2009
Source: Wang, W.,Li, Z.,Wang, D.I.C. (2009). Immobilization of enzymes on functionalized magnetic nanoparticles for efficient biocatalysis. Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 2 : 337-339. ScholarBank@NUS Repository.
Abstract: In this paper, iron oxide magnetic nanoparticles were synthesized by co-precipitation method and were functionalized with aldehyde group. Spherical superparamagnetic nanoparticles with diameter of 100 nm were obtained based on the results of Held emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). These particles were further applied for immobilization of enzymes by covalent binding between aldehyde group on particles and amino group on the enzyme surface. Different pH was applied for optimization of the immobilization conditions. The result showed that pH 4.0 is the best pH for immobilizing BSA on the nanomctric support.
Source Title: Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
URI: http://scholarbank.nus.edu.sg/handle/10635/74620
ISBN: 9781439817834
Appears in Collections:Staff Publications

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