Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/74613
Title: Growing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microsphere scaffold
Authors: Zhu, X.H. 
Wang, C.-H. 
Tong, Y.W. 
Keywords: Liver cells
Liver tissue engineering
Microspheres
PHBV
Issue Date: 2006
Source: Zhu, X.H.,Wang, C.-H.,Tong, Y.W. (2006). Growing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microsphere scaffold. AIChE Annual Meeting, Conference Proceedings : -. ScholarBank@NUS Repository.
Abstract: In this study, a solvent evaporation technique was used to fabricate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV, 8% PHV) microspheres as scaffold to guide liver cell growth. Human hepatoma cell lines, HepG2 and Hep3B, were cultured in vitro on both the microspheres and polymer films. SEM and optical microscope images showed that multilayer cells were formed among the microspheres to bridge them together and developed into cell-construct aggregates after one week of culture. MTT results showed that the cell proliferation on the microspheres was more than two times higher than that on the films after 12 days of culture. The cells seeded on microspheres secreted albumin 2-4 times more than that on the positive control after one weeks of culture, which indicated that this hepatic function was greatly improved by the aggregation of cells on microspheres. Although HepG2 failed to express P-450 activity, this hepatic function was preserved when Hep3B cultured on microspheres. All the results indicated that PHBV microspheres are appropriate scaffolds for liver tissue engineering.
Source Title: AIChE Annual Meeting, Conference Proceedings
URI: http://scholarbank.nus.edu.sg/handle/10635/74613
ISBN: 081691012X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

26
checked on Dec 9, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.