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|Title:||Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst|
Functionalization of mesoporous silicas
Penicillin G acylase
|Citation:||Chong, A.S.M., Zhao, X.S. (2004-09-01). Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst. Catalysis Today 93-95 : 293-299. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cattod.2004.06.064|
|Abstract:||In this study, functionalization of large-pore nanoporous silica materials was carried out by condensation of tetraethylorthosilicate (TEOS) and 3-aminopropyltriethoxysilane (APTES), 3-mercaptopropyltrimethoxysilane (MPTMS), phenyltrimethoxysilane (PTMS), vinyltriethoxysilane (VTES), and 4-(triethoxysilyl)butyronitrile (TSBN), respectively, in the presence of non-ionic surfactant under acidic conditions. The TSBN functionality was subsequently converted to carboxyl group while APTES was further functionalized with glutardialdehyde, a cross linker. The various functionalized materials were used as supports for immobilization of enzyme penicillin G acylase (PGA). Experimental data showed that the functionalized materials except for the material functionalized with MPTMS possess a faster loading kinetics and a higher loading amount of enzyme PGA than the pure-silica counterpart. The enzymatic catalytic activities of the immobilized biocatalysts varied from 52.2 to 167.5U/g of solid. The glutardialdehyde-activated material displayed the highest initial immobilized enzyme activity and the most stable activity among all the support materials. PGA immobilized on VTES-functionalized nanoporous silica showed the highest initial enzymatic activity (67.7U/mg of PGA, much higher than that of free PGA (300U/mg of PGA). Experimental data along with theoretical analysis results indicate that glutardialdehyde is a good cross linker, offering covalent binding of PGA with the support materials while VTES-functionalized nanoporous silica is a very good potential support for physical entrapment of PGA enzyme. © 2004 Elsevier B.V. All rights reserved.|
|Source Title:||Catalysis Today|
|Appears in Collections:||Staff Publications|
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