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|Title:||High-throughput circulating tumor cells (CTCs) isolation using inertial forces|
|Keywords:||Circulating tumor cells|
|Citation:||Bhagat, A.A.S.,Hou, H.W.,Huang, S.,Lim, C.T.,Han, J. (2010). High-throughput circulating tumor cells (CTCs) isolation using inertial forces. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2 : 1391-1393. ScholarBank@NUS Repository.|
|Abstract:||Continuous flow-based cell separations are indispensable for many biomedical applications and diagnostics including early detection of cancer. Here, we describe a high-throughput cell separation technique for sorting circulating tumor cells (CTCs) from blood using microfluidics. The separation consists of a 2-step process to efficiently isolate CTCs from blood. In the first step, 99% of hematologic cells (erythrocytes (RBCs) and leukocytes) are removed using shear-modulated inertial focusing with >90% CTC recovery. This RBCs depleted sample is then run through a low aspect ratio spiral microchannel to completely isolate the CTCs. The operating principle of this separation method exploits the difference in cell size between CTCs (∼16-20 μm diameter) and other blood cells (RBCs ∼8 μm; leukocytes ∼8-14 μm).|
|Source Title:||14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010|
|Appears in Collections:||Staff Publications|
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