Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/73310
Title: Deformability based cell margination for malarial infected red blood cell enrichment
Authors: Hou, H.W.
Asgar, A.
Bhagat, S.
Mao, P.
Han, J.
Lim, C.T. 
Keywords: Cell separation
Deformability
Malaria
Margination
Red blood cells
Issue Date: 2010
Source: Hou, H.W.,Asgar, A.,Bhagat, S.,Mao, P.,Han, J.,Lim, C.T. (2010). Deformability based cell margination for malarial infected red blood cell enrichment. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2 : 1370-1372. ScholarBank@NUS Repository.
Abstract: In this work, the physiological phenomenon of leukocyte margination is mimicked in microfluidic systems for biological separation of malarial infected RBCs (iRBCs) from blood. Change in cell stiffness is a characteristic of iRBCs which can act as an intrinsic biomarker for separation. Tests were conducted using early ring stage and late trophozoite/schizont stage iRBCs which vary significantly in their deformability. Filtration efficiency was quantified by analyzing the dispersion of iRBCs across the microchannel width at the outlet. Flow cytometry analysis was conducted on the outlet samples indicating filtration efficiency of ∼80% for early stage iRBCs and >90% for late stage iRBCs. This is the first demonstration applying this unique biomimetic separation technique to iRBCs filtration for disease diagnostic application.
Source Title: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
URI: http://scholarbank.nus.edu.sg/handle/10635/73310
ISBN: 9781618390622
Appears in Collections:Staff Publications

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