Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.addr.2011.03.003
Title: Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies
Authors: Chen, C. 
Loe, F. 
Blocki, A.
Peng, Y.
Raghunath, M. 
Keywords: Collagen assembly
Differentiated cells
Excluded volume effect
Matrix maturation
Microenvironment
Stem cells
Issue Date: 30-Apr-2011
Source: Chen, C., Loe, F., Blocki, A., Peng, Y., Raghunath, M. (2011-04-30). Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies. Advanced Drug Delivery Reviews 63 (4) : 277-290. ScholarBank@NUS Repository. https://doi.org/10.1016/j.addr.2011.03.003
Abstract: With the advent of multicellular organisms, the exterior of the cells evolved dramatically from highly aqueous surroundings into an extracellular matrix and space crowded with macromolecules. Cell-based therapies require removal of cells from their crowded physiological context and propagating them in dilute culture medium to attain therapeutically relevant numbers whilst preserving their phenotype. However, bereft of their microenvironment, cells under perform and lose functionality. Major efforts currently aim to modify cell culture surfaces and build three dimensional scaffolds to improve this situation. We discuss here alternative strategies that enable cells to re-create their own microenvironment in vitro, using carbohydrate-based macromolecules as culture media additives that create an excluded volume effect at defined fraction volume occupancies. This biophysical approach dramatically enhances extracellular matrix deposition by differentiated cells and stem cells, and boosts progenitor cell differentiation and proliferation. We begin to understand how well cells really can perform ex vivo if given the chance. © 2011 Elsevier B.V.
Source Title: Advanced Drug Delivery Reviews
URI: http://scholarbank.nus.edu.sg/handle/10635/68134
ISSN: 0169409X
DOI: 10.1016/j.addr.2011.03.003
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