Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1742-4658.2007.05638.x
Title: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis
Authors: Ang, E.L.
Obbard, J.P. 
Zhao, H.
Keywords: Aniline dioxygenase
Homology modeling
Saturation mutagenesis
Substrate specificity
Issue Date: Feb-2007
Source: Ang, E.L., Obbard, J.P., Zhao, H. (2007-02). Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis. FEBS Journal 274 (4) : 928-939. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1742-4658.2007.05638.x
Abstract: Aniline dioxygenase is a multicomponent Rieske nonheme-iron dioxygenase enzyme isolated from Acinetobacter sp. strain YAA. Saturation mutagenesis of the substrate-binding pocket residues, which were identified using a homology model of the α subunit of the terminal dioxygenase (AtdA3), was used to probe the molecular determinants of AtdA substrate specificity. The V205A mutation widened the substrate specificity of aniline dioxygenase to include 2-isopropylaniline, for which the wild-type enzyme has no activity. The V205A mutation also made 2-isopropylaniline a better substrate for the enzyme than 2,4-dimethylaniline, a native substrate of the wild-type enzyme. The I248L mutation improved the activity of aniline dioxygenase against aniline and 2,4-dimethylaniline approximately 1.7-fold and 2.1-fold, respectively. Thus, it is shown that the α subunit of the terminal dioxygenase indeed plays a part in the substrate specificity as well as the activity of aniline dioxygenase. Interestingly, the equivalent residues of V205 and I248 have not been previously reported to influence the substrate specificity of other Rieske dioxygenases. These results should facilitate future engineering of the enzyme for bioremediation and industrial applications. © 2007 The Authors.
Source Title: FEBS Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/67685
ISSN: 1742464X
DOI: 10.1111/j.1742-4658.2007.05638.x
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