Please use this identifier to cite or link to this item:
Title: Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines
Authors: Ang, E.L.
Obbard, J.P. 
Zhao, H.
Keywords: Aniline dioxygenase
Random mutagenesis
Saturation mutagenesis
Substrate specificity
Issue Date: Jan-2009
Citation: Ang, E.L., Obbard, J.P., Zhao, H. (2009-01). Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines. Applied Microbiology and Biotechnology 81 (6) : 1063-1070. ScholarBank@NUS Repository.
Abstract: The objective of this study was to enhance the activity of aniline dioxygenase (AtdA), a multi-component Rieske non-heme iron dioxygenase enzyme isolated from Acinetobacter sp. strain YAA, so as to create an enhanced biocatalyst for the bioremediation of aromatic amines. Previously, the mutation V205A was found to widen the substrate specificity of AtdA to accept 2-isopropylaniline (2IPA) for which the wild-type enzyme has no activity (Ang EL, Obbard JP, Zhao HM, FEBS J, 274:928-939, 2007). Using mutant V205A as the parent and applying one round of saturation mutagenesis followed by a round of random mutagenesis, the activity of the final mutant, 3-R21, was increased by 8.9-, 98.0-, and 2.0-fold for aniline, 2,4-dimethylaniline (24DMA), and 2-isopropylaniline (2IPA), respectively, over the mutant V205A. In particular, the activity of the mutant 3-R21 for 24DMA, which is a carcinogenic aromatic amine pollutant, was increased by 3.5-fold over the wild-type AtdA, while the AN activity was restored to the wild-type level, thus yielding a mutant aniline dioxygenase with enhanced activity and capable of hydroxylating a wider range of aromatic amines than the wild type. © 2008 Springer-Verlag.
Source Title: Applied Microbiology and Biotechnology
ISSN: 01757598
DOI: 10.1007/s00253-008-1710-0
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 22, 2019


checked on Feb 5, 2019

Page view(s)

checked on Jan 12, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.