Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.jsb.2010.05.007
DC Field | Value | |
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dc.title | Crystal structure of a fructokinase homolog from Halothermothrix orenii | |
dc.contributor.author | Chua, T.K. | |
dc.contributor.author | Seetharaman, J. | |
dc.contributor.author | Kasprzak, J.M. | |
dc.contributor.author | Ng, C. | |
dc.contributor.author | Patel, B.K.C. | |
dc.contributor.author | Love, C. | |
dc.contributor.author | Bujnicki, J.M. | |
dc.contributor.author | Sivaraman, J. | |
dc.date.accessioned | 2014-06-17T10:14:37Z | |
dc.date.available | 2014-06-17T10:14:37Z | |
dc.date.issued | 2010-09 | |
dc.identifier.citation | Chua, T.K., Seetharaman, J., Kasprzak, J.M., Ng, C., Patel, B.K.C., Love, C., Bujnicki, J.M., Sivaraman, J. (2010-09). Crystal structure of a fructokinase homolog from Halothermothrix orenii. Journal of Structural Biology 171 (3) : 397-401. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jsb.2010.05.007 | |
dc.identifier.issn | 10478477 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67629 | |
dc.description.abstract | Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mechanism of FRK, here we report the crystal structure of a FRK homolog from a thermohalophilic bacterium Halothermothrix orenii (Hore_18220 in sequence databases). The structure of the Hore_18220 protein reveals a catalytic domain with a Rossmann-like fold and a β-sheet " lid" for dimerization. Based on comparison of Hore_18220 to structures of related proteins, we propose its mechanism of action, in which the lid serves to regulate access to the substrate binding sites. Close relationship of Hore_18220 and plant FRK enzymes allows us to propose a model for the structure and function of FRKs. © 2010 Elsevier Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jsb.2010.05.007 | |
dc.source | Scopus | |
dc.subject | ATP | |
dc.subject | FRK homolog | |
dc.subject | Fructokinase | |
dc.subject | Fructose | |
dc.subject | Hore_18220 | |
dc.subject | Structure | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | DIVISION OF ENVIRONMENTAL SCIENCE & ENGG | |
dc.description.doi | 10.1016/j.jsb.2010.05.007 | |
dc.description.sourcetitle | Journal of Structural Biology | |
dc.description.volume | 171 | |
dc.description.issue | 3 | |
dc.description.page | 397-401 | |
dc.description.coden | JSBIE | |
dc.identifier.isiut | 000280680100017 | |
Appears in Collections: | Staff Publications |
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