Please use this identifier to cite or link to this item:
https://doi.org/10.1264/jsme2.ME09109
DC Field | Value | |
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dc.title | Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewater | |
dc.contributor.author | Chen, C.-L. | |
dc.contributor.author | Wu, J.-H. | |
dc.contributor.author | Tseng, I.-C. | |
dc.contributor.author | Liang, T.-M. | |
dc.contributor.author | Liu, W.-T. | |
dc.date.accessioned | 2014-06-17T10:14:33Z | |
dc.date.available | 2014-06-17T10:14:33Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Chen, C.-L., Wu, J.-H., Tseng, I.-C., Liang, T.-M., Liu, W.-T. (2009). Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewater. Microbes and Environments 24 (2) : 144-153. ScholarBank@NUS Repository. https://doi.org/10.1264/jsme2.ME09109 | |
dc.identifier.issn | 13426311 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67624 | |
dc.description.abstract | This study investigated the active microbial community in a full-scale granular activated carbon-anaerobic fluidized bed (GAC-AFB) reactor treating wastewater from the manufacturing of phenolic resin, using 16S rRNA-based molecular analyses. The results of cDNA from 16S rRNA revealed that Methanosaeta-related (83.9% of archaeal clones) and Syntrophorhabdaceae (formerly named Deltaproteobacteria group TA)-related (68.9% of bacterial clones) microorganisms were as the most predominant populations in the phenol-degrading GAC-AFB reactor. The high abundance of Syntrophorhabdaceae was supported by a terminal restriction fragment length polymorphism (T-RFLP) analysis, which showed that a Syntrophorhabdaceae-like fragment of 119 bp (∼80% of total fragments) was the most predominant phylotype. Furthermore, fluorescence in situ hybridization (FISH) analyses suggested that Syntrophus-and Chloroflexi-like cells were also in high abundance in the GAC biofilm. A non-layered structure of microorganisms was found in the GAC biofilm, where Methanosaeta (thick filamentous), Syntrophorhabdaceae (oval-shaped), Syntrophus (small rods) and Chloroflexi (thin-filamentous) were randomly distributed with high abundance. These findings greatly improve our understanding of the diversity and distribution of microbial populations in a full-scale mesophilic bioreactor treating an actual phenol-containing waste stream. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1264/jsme2.ME09109 | |
dc.source | Scopus | |
dc.subject | Anaerobic | |
dc.subject | FISH | |
dc.subject | Microbial | |
dc.subject | Phenol | |
dc.subject | Phylogenetic | |
dc.type | Article | |
dc.contributor.department | DIVISION OF ENVIRONMENTAL SCIENCE & ENGG | |
dc.contributor.department | TROPICAL MARINE SCIENCE INSTITUTE | |
dc.description.doi | 10.1264/jsme2.ME09109 | |
dc.description.sourcetitle | Microbes and Environments | |
dc.description.volume | 24 | |
dc.description.issue | 2 | |
dc.description.page | 144-153 | |
dc.identifier.isiut | 000273264500009 | |
Appears in Collections: | Staff Publications |
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