Please use this identifier to cite or link to this item: https://doi.org/10.1264/jsme2.ME09109
Title: Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewater
Authors: Chen, C.-L. 
Wu, J.-H.
Tseng, I.-C.
Liang, T.-M.
Liu, W.-T. 
Keywords: Anaerobic
FISH
Microbial
Phenol
Phylogenetic
Issue Date: 2009
Source: Chen, C.-L., Wu, J.-H., Tseng, I.-C., Liang, T.-M., Liu, W.-T. (2009). Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewater. Microbes and Environments 24 (2) : 144-153. ScholarBank@NUS Repository. https://doi.org/10.1264/jsme2.ME09109
Abstract: This study investigated the active microbial community in a full-scale granular activated carbon-anaerobic fluidized bed (GAC-AFB) reactor treating wastewater from the manufacturing of phenolic resin, using 16S rRNA-based molecular analyses. The results of cDNA from 16S rRNA revealed that Methanosaeta-related (83.9% of archaeal clones) and Syntrophorhabdaceae (formerly named Deltaproteobacteria group TA)-related (68.9% of bacterial clones) microorganisms were as the most predominant populations in the phenol-degrading GAC-AFB reactor. The high abundance of Syntrophorhabdaceae was supported by a terminal restriction fragment length polymorphism (T-RFLP) analysis, which showed that a Syntrophorhabdaceae-like fragment of 119 bp (∼80% of total fragments) was the most predominant phylotype. Furthermore, fluorescence in situ hybridization (FISH) analyses suggested that Syntrophus-and Chloroflexi-like cells were also in high abundance in the GAC biofilm. A non-layered structure of microorganisms was found in the GAC biofilm, where Methanosaeta (thick filamentous), Syntrophorhabdaceae (oval-shaped), Syntrophus (small rods) and Chloroflexi (thin-filamentous) were randomly distributed with high abundance. These findings greatly improve our understanding of the diversity and distribution of microbial populations in a full-scale mesophilic bioreactor treating an actual phenol-containing waste stream.
Source Title: Microbes and Environments
URI: http://scholarbank.nus.edu.sg/handle/10635/67624
ISSN: 13426311
DOI: 10.1264/jsme2.ME09109
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