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Title: Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging
Authors: Kou, S.S.
Waller, L.
Barbastathis, G.
Sheppard, C.J.R. 
Issue Date: 1-Feb-2010
Citation: Kou, S.S.,Waller, L.,Barbastathis, G.,Sheppard, C.J.R. (2010-02-01). Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. Optics Letters 35 (3) : 447-449. ScholarBank@NUS Repository.
Abstract: Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values © 2010 Optical Society of America.
Source Title: Optics Letters
ISSN: 01469592
DOI: 10.1364/OL.35.000447
Appears in Collections:Staff Publications

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