Please use this identifier to cite or link to this item:
|Title:||Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers|
|Citation:||Bao, H., Ping, Y., Pan, Y., Li, L., Li, J., Gan, L.H. (2012-02-28). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter 8 (8) : 2518-2526. ScholarBank@NUS Repository. https://doi.org/10.1039/c2sm07083a|
|Abstract:||Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5-35.1°C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37°C for 22 h, 20°C for 2 h and 37°C for 24 h increased 1-4 fold compared to that incubated at 37°C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of "gold-standard" PEI (25 kDa). © 2012 The Royal Society of Chemistry.|
|Source Title:||Soft Matter|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 17, 2018
WEB OF SCIENCETM
checked on Jun 27, 2018
checked on May 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.