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Title: Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers
Authors: Bao, H.
Ping, Y.
Pan, Y.
Li, L.
Li, J. 
Gan, L.H.
Issue Date: 28-Feb-2012
Citation: Bao, H., Ping, Y., Pan, Y., Li, L., Li, J., Gan, L.H. (2012-02-28). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter 8 (8) : 2518-2526. ScholarBank@NUS Repository.
Abstract: Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5-35.1°C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37°C for 22 h, 20°C for 2 h and 37°C for 24 h increased 1-4 fold compared to that incubated at 37°C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of "gold-standard" PEI (25 kDa). © 2012 The Royal Society of Chemistry.
Source Title: Soft Matter
ISSN: 1744683X
DOI: 10.1039/c2sm07083a
Appears in Collections:Staff Publications

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