Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nano.2009.11.004
Title: Singlet oxygen-induced apoptosis of cancer cells using upconversion fluorescent nanoparticles as a carrier of photosensitizer
Authors: Guo, H. 
Qian, H. 
Idris, N.M. 
Zhang, Y. 
Keywords: Apoptosis
Mesoporous silica
Photodynamic
Singlet oxygen
Upconversion
Issue Date: Jun-2010
Source: Guo, H.,Qian, H.,Idris, N.M.,Zhang, Y. (2010-06). Singlet oxygen-induced apoptosis of cancer cells using upconversion fluorescent nanoparticles as a carrier of photosensitizer. Nanomedicine: Nanotechnology, Biology, and Medicine 6 (3) : 486-495. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nano.2009.11.004
Abstract: The photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was studied on murine bladder cancer cells (MB49). Mesoporous silica was coated onto sodium yttrium fluoride upconversion nanocrystals to form a core-shell structure and then loaded with the photosensitizer zinc (II)-phthalocyanine into the porous silica. The nanoparticles displayed a uniform spherical shape with an average diameter of about 50 nm and showed good dispersibility in water. Intracellular uptake study in MB49 cells revealed a time- and concentration-dependent accumulation of these nanoparticles. Upon irradiation with 980-nm near-infrared light, their efficiency in activating the loaded zinc (II)-phthalocyanine to generate singlet oxygen molecules was confirmed in live cells. The cytotoxic effect of the released singlet oxygen from the nanoplatform was proven by cell viability assay, confocal microscopy, DNA agarose gel electrophoresis, cytochrome c-releasing assay, and prostate-specific antigen-enzyme-linked immunosorbent assay, all of which showed a strong photodynamic effect of the nanoparticles on MB49 cells. This suggests the efficacy of sodium yttrium fluoride upconversion nanoparticles as a carrier for photosensitizers and their use in photodynamic therapy of cancer and some other diseases. From the Clinical Editor: In this study, the photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was investigated on murine bladder cancer cells, with strongly positive results, which may pave its way to future clinical use in malignant tumors and potentially other diseases. © 2010 Elsevier Inc. All rights reserved.
Source Title: Nanomedicine: Nanotechnology, Biology, and Medicine
URI: http://scholarbank.nus.edu.sg/handle/10635/67278
ISSN: 15499634
DOI: 10.1016/j.nano.2009.11.004
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