Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aca.2007.11.046
Title: Reusable optical bioassay platform with permeability-controlled hydrogel pads for selective saccharide detection
Authors: Cheung, K.Y. 
Mak, W.C. 
Trau, D. 
Keywords: Hydrogel pad
Layer-by-Layer
Optical bioassay
Permeability control
Saccharide detection
Issue Date: 28-Jan-2008
Source: Cheung, K.Y., Mak, W.C., Trau, D. (2008-01-28). Reusable optical bioassay platform with permeability-controlled hydrogel pads for selective saccharide detection. Analytica Chimica Acta 607 (2) : 204-210. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aca.2007.11.046
Abstract: A reusable optical bioassay platform using permeability-controlled hydrogel pads for selective saccharide detection has been developed. An optical glucose detection assay based on fluorescence resonance energy transfer (FRET) between dye-labeled dextran and Concanavalin A (ConA) was incorporated into hydrogel pads by entrapment. The hydrogel pads are constructed from hemispherical hydrogel attached onto hydrophobic surfaces of a microtiter plate. The resulted hemispherical hydrogel pads entrapping the sensing biological materials were further surface coated with polyelectrolyte multilayers through a Layer-by-Layer (LbL) self-assembly process to create a permeability-controlled membrane with nanometer thickness. The selective permeable LbL film deposited on the hydrogel surface allows small molecular weight analytes to diffuse into the hydrogel pads while the large molecular weight sensing biological molecules are immobilized. An encapsulation efficiency of 75% for the ConA/Dextran complex within the coated hydrogel pads was achieved and no significant leakage of the complex was observed. Glucose calibration curve with linear range from 0 to 10 mM glucose was obtained. Selective permeability of the hydrogel pads has been demonstrated by measurement of saccharides with various molecular weights. The LbL hydrogel pads could selectively detect monosaccharides (glucose, MW = 180) and disaccharides (sucrose, MW = 342) while polysaccharides (dextran, MW ∼ 70 kDa) cannot diffuse through the LbL layer and are excluded. LbL hydrogel pads allow regeneration of the FRET system with good signal reproducibility of more than 90% to construct a reusable and reagentless optical bioassay platform. © 2007 Elsevier B.V. All rights reserved.
Source Title: Analytica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/67258
ISSN: 00032670
DOI: 10.1016/j.aca.2007.11.046
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

12
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

11
checked on Nov 22, 2017

Page view(s)

39
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.