Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.3374051
Title: Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophore characterization using an acousto-optic tunable filter technique
Authors: Li, H. 
Zheng, W. 
Huang, Z. 
Keywords: Acousto-optic tunable filter (AOTF)
Excitation-emission matrices (EEMS)
Fluorescence
Near-infrared (NIR) fluorescence
Spectroscopy
Issue Date: 2010
Source: Li, H., Zheng, W., Huang, Z. (2010). Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophore characterization using an acousto-optic tunable filter technique. Journal of Biomedical Optics 15 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1117/1.3374051
Abstract: We report on a novel acousto-optic tunable filter (AOTF)-based near-infrared (NIR) fluorescence excitation-emission matrix (EEM) spectroscopy technique for rapid multifluorophore characterization. We implement a unique light filtering module design by using cascaded AOTFs coupled with three orthogonally oriented polarizers to effectively remove the side-ripple artifacts of AOTFs as well as by using a pair of AOTFs coupled with two orthogonally oriented polarizers to improve detection efficiency for high-quality fluorescence EEM acquisitions. NIR fluorescence EEM spectroscopy (41 excitation wavelengths ranging from 550 to 950 nm in 10-nm increments; fluorescence emission from 570 to 1000 nm at 10-nm intervals) can be acquired from fluorescence dyes [e.g., diethylthiatricarbocyanine (DTTC) iodide, oxazine 750, and IR 140] within 10 s or even less, illustrating the potential of the AOTF-based NIR EEM technique developed for rapid multifluorophore analysis and characterization in biochemical and biomedical systems. © 2010 Society of Photo-Optical Instrumentation Engineers.
Source Title: Journal of Biomedical Optics
URI: http://scholarbank.nus.edu.sg/handle/10635/67251
ISSN: 10833668
DOI: 10.1117/1.3374051
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