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|Title:||Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast|
|Citation:||Mehta, S.B.,Sheppard, C.J.R. (2009-07-01). Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast. Optics Letters 34 (13) : 1924-1926. ScholarBank@NUS Repository. https://doi.org/10.1364/OL.34.001924|
|Abstract:||We describe a full-field phase-gradient imaging method: asymmetric illumination-based differential phase contrast (AIDPC). Imaging properties of AIDPC are evaluated using the phase-gradient transfer-function approach and elucidated with experimental images of an optical fiber and a histochemical preparation of a skeletal muscle section. In comparison with full-field differential interference contrast, AIDPC does not require phase shifting for quantitative imaging of phase gradient, provides artifact-free images of birefringent specimens, requires shorter camera exposure, and has larger depth of focus. It is amenable to transferfunction engineering, simultaneous fluorescence imaging, and automated live cell imaging. © 2009 Optical Society of America.|
|Source Title:||Optics Letters|
|Appears in Collections:||Staff Publications|
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