Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bbrc.2008.11.127
DC Field | Value | |
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dc.title | Pharmacologically induced angiogenesis in transgenic zebrafish | |
dc.contributor.author | Raghunath, M. | |
dc.contributor.author | Sy Wong, Y. | |
dc.contributor.author | Farooq, M. | |
dc.contributor.author | Ge, R. | |
dc.date.accessioned | 2014-06-17T09:45:50Z | |
dc.date.available | 2014-06-17T09:45:50Z | |
dc.date.issued | 2009-01-23 | |
dc.identifier.citation | Raghunath, M., Sy Wong, Y., Farooq, M., Ge, R. (2009-01-23). Pharmacologically induced angiogenesis in transgenic zebrafish. Biochemical and Biophysical Research Communications 378 (4) : 766-771. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.11.127 | |
dc.identifier.issn | 0006291X | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67216 | |
dc.description.abstract | The rapid vascularisation of biomaterials and engineered tissue after implantation is a current unmet need. To this end, we explored the pharmacological option of inducing neovascularisation using compounds that inhibit hypoxia-induced factor-1α prolyl hydroxylase. This stabilises hypoxia inducible factor-1α and therefore de-repress the transcription of various angiogenic genes. In the quest for a small vertebrate model allowing for in vivo screening we exposed (TG(Fli1:EGFP)) transgenic zebrafish embryos exhibiting fluorescent blood vessels to hydralazine hydrochloride and 2,4-pyridine dicarboxylic acid from 6 hpf to 72 hpf by immersion. Live observation of embryos revealed that the substances induced formation of ectopic blood vessels in the subintestinal vessel basket. We confirmed the HIF-stabilising effects biochemically in human fibroblasts and with an in vitro angiogenesis fibroblast/HUVEC co-culture model. Cross-inhibition of collagen prolyl hydroxylase was confirmed by reduced collagen secretion by fibroblasts and reduced collagen content of zebrafish embryos. © 2008 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2008.11.127 | |
dc.source | Scopus | |
dc.subject | Collagen | |
dc.subject | Drug screening | |
dc.subject | Fli1 | |
dc.subject | Hypoxia-induced factor-1α | |
dc.subject | Neovascularisation | |
dc.subject | Prolyl hydroxylase inhibitors | |
dc.subject | Subintestinal vessels | |
dc.subject | Zebrafish | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | BIOENGINEERING | |
dc.description.doi | 10.1016/j.bbrc.2008.11.127 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 378 | |
dc.description.issue | 4 | |
dc.description.page | 766-771 | |
dc.description.coden | BBRCA | |
dc.identifier.isiut | 000262447400016 | |
Appears in Collections: | Staff Publications |
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