Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2007.07.112
Title: Osteo-maturation of adipose-derived stem cells required the combined action of vitamin D3, β-glycerophosphate, and ascorbic acid
Authors: Gupta, A.
Leong, D.T. 
Bai, H.F.
Singh, S.B.
Lim, T.-C.
Hutmacher, D.W. 
Keywords: Adipose-derived stem cells
Differentiation
Induction
Osteoblasts
Vitamin D3
Issue Date: 12-Oct-2007
Source: Gupta, A.,Leong, D.T.,Bai, H.F.,Singh, S.B.,Lim, T.-C.,Hutmacher, D.W. (2007-10-12). Osteo-maturation of adipose-derived stem cells required the combined action of vitamin D3, β-glycerophosphate, and ascorbic acid. Biochemical and Biophysical Research Communications 362 (1) : 17-24. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2007.07.112
Abstract: This study investigated the effects of various components [vitamin D3 (VD3), β-glycerophosphate (BGP), and ascorbic acid (AA)] on the potential of human adipose-derived progenitor cells (ADPCs) to transdifferentiate into osteoblast-like cells. ADPCs were induced under four different supplement groups: (1) VD3 + BGP + AA, (2) VD3 alone, (3) BGP + AA, and (4) no VD3, BGP or AA. Mineralization studies and presence of bone matrix-related proteins by immunostaining showed that the Group 1 ADPCs showed their ability to undergo osteoblastic differentiation. Further evaluation was made by estimation of levels of RUNX-2 and TAZ genes. Group 1 ADPCs showed the consistent expression of RUNX-2 and TAZ levels over the study period of 28 days. The study showed good correlation among various parameters evaluated to conclude that ADPCs could be an alternative source for generating osteoblast-like cells. © 2007 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/67208
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2007.07.112
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