Please use this identifier to cite or link to this item:
|Title:||Osteogenic and adipogenic induction potential of human periodontal cells|
|Authors:||Zhou, Y. |
|Citation:||Zhou, Y., Hutmacher, D.W., Sae-Lim, V., Zhou, Z., Woodruff, M., Lim, T.M. (2008-03). Osteogenic and adipogenic induction potential of human periodontal cells. Journal of Periodontology 79 (3) : 525-534. ScholarBank@NUS Repository. https://doi.org/10.1902/jop.2008.070373|
|Abstract:||Background: Human periodontium contains different cell types that have various potential roles in hard and soft tissue regeneration. However, there is limited knowledge about how these diverse cell populations contribute to the regenerative process. In this study, we investigated the surface marker difference between different periodontal cells (alveolar osteoblasts [AOs], periodontal ligament fibroblasts [PDLFs], and gingival fibroblasts [GFs] ) and their differentiation potential to-ward osteogenic and adipogenic phenotypes. Methods: Periodontal cells (AOs, PDLFs, and GFs) from 14 subjects were isolated. The surface antigen expression pattern of cells was analyzed by cell flow cytometry, and the molecular and histologic characterizations under osteogenic and adipogenic inductions were monitored by reverse transcriptionpolymerase chain reaction, Western blot, and immunocytohistology. Results: The cell phenotypes of AOs were verified by the high expressions of CD29 and CD49a, whereas PDLFs showed distinctively low levels of CD63 and CD73. Under adipogenic induction, limited AOs formed cube-shaped adipose-like cells, whereas PDLFs formed spindle-shaped adipose-like cells. All three cell types expressed baseline osteo-related genes. AOs demonstrated the highest osteogenic ability followed by PDLFs and GFs. Conclusions: Cells in alveolar bone and periodontal ligament contain osteogenic and adipogenic progenitors. These observations indicate a possible application for periodontium cells in hard or soft tissue regeneration.|
|Source Title:||Journal of Periodontology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on May 1, 2018
checked on May 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.