Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.JBO.18.6.067007
Title: Near-infrared-excited confocal Raman spectroscopy advances in vivo diagnosis of cervical precancer
Authors: Duraipandian, S.
Zheng, W. 
Ng, J.
Low, J.J.H.
Ilancheran, A.
Huang, Z. 
Keywords: autofluorescence
cervical dysplasia
confocal Raman spectroscopy
in vivo diagnosis
near-infrared
Issue Date: 2013
Source: Duraipandian, S., Zheng, W., Ng, J., Low, J.J.H., Ilancheran, A., Huang, Z. (2013). Near-infrared-excited confocal Raman spectroscopy advances in vivo diagnosis of cervical precancer. Journal of Biomedical Optics 18 (6) : -. ScholarBank@NUS Repository. https://doi.org/10.1117/1.JBO.18.6.067007
Abstract: Raman spectroscopy is a unique optical technique that can probe the changes of vibrational modes of biomolecules associated with tissue premalignant transformation. This study evaluates the clinical utility of confocal Raman spectroscopy over near-infrared (NIR) autofluorescence (AF) spectroscopy and composite NIR AF/Raman spectroscopy for improving early diagnosis of cervical precancer in vivo at colposcopy. A rapid NIR Raman system coupled with a ball-lens fiber-optic confocal Raman probe was utilized for in vivo NIR AF/Raman spectral measurements of the cervix. A total of 1240 in vivo Raman spectra [normal (n=993), dysplasia (n=247)] were acquired from 84 cervical patients. Principal components analysis (PCA) and linear discriminant analysis (LDA) together with a leave-one-patient-out, cross-validation method were used to extract the diagnostic information associated with distinctive spectroscopic modalities. The diagnostic ability of confocal Raman spectroscopy was evaluated using the PCA-LDA model developed from the significant principal components (PCs) [i.e., PC4, 0.0023%; PC5, 0.00095%; PC8, 0.00022%, (p<0.05)], representing the primary tissue Raman features (e.g., 854, 937, 1095, 1253, 1311, 1445, and 1654 cm-1). Confocal Raman spectroscopy coupled with PCA-LDA modeling yielded the diagnostic accuracy of 84.1% (a sensitivity of 81.0% and a specificity of 87.1%) for in vivo discrimination of dysplastic cervix. The receiver operating characteristic curves further confirmed that the best classification was achieved using confocal Raman spectroscopy compared to the composite NIR AF/Raman spectroscopy or NIR AF spectroscopy alone. This study illustrates that confocal Raman spectroscopy has great potential to improve early diagnosis of cervical precancer in vivo during clinical colposcopy. © 2013 SPIE.
Source Title: Journal of Biomedical Optics
URI: http://scholarbank.nus.edu.sg/handle/10635/67190
ISSN: 10833668
DOI: 10.1117/1.JBO.18.6.067007
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