Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2005.07.008
Title: Nanoparticle-assisted micropatterning of active proteins on solid substrate
Authors: Wang, C. 
Zhang, Y. 
Seng, H.S.
Ngo, L.L.
Keywords: Micropatterning
Nanoparticle
Protein
Silica
Issue Date: 15-Feb-2006
Source: Wang, C., Zhang, Y., Seng, H.S., Ngo, L.L. (2006-02-15). Nanoparticle-assisted micropatterning of active proteins on solid substrate. Biosensors and Bioelectronics 21 (8) : 1638-1643. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2005.07.008
Abstract: Micropatterning of proteins on silica substrate was achieved using a new method. Proteins were first immobilized onto silica nanoparticles which were then dispensed into arrayed microwells on silicon. Atomic force microscopy (AFM), fluorescence microscopy and Fourier transform infrared (FTIR) spectroscopy were used to characterize the samples. The results showed that, compared to a planar surface, curved surfaces of nanoparticles provide more space for attaching proteins and thus increases the intensity of fluorescence signal. Furthermore, after attaching to silica nanoparticles, bovine serum albumin (BSA) maintains its major structure and the cytokine IFN-γ maintains its ability to bind to its antibody. Use of this method can be extended to micropatterning of other biomolecules, such as DNA and enzymes. © 2005 Elsevier B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/67185
ISSN: 09565663
DOI: 10.1016/j.bios.2005.07.008
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