Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2009.11.012
Title: Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-γ-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector
Authors: Huang, H.
Yu, H.
Tang, G.
Wang, Q.
Li, J. 
Keywords: Cancer gene therapy
Cyclodextrin
HER2
Non-viral gene vector
Polyethylenimine
Issue Date: Mar-2010
Source: Huang, H., Yu, H., Tang, G., Wang, Q., Li, J. (2010-03). Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-γ-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector. Biomaterials 31 (7) : 1830-1838. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.11.012
Abstract: Gene delivery is one of the critical steps for gene therapy. Non-viral vectors have many advantages but suffered from low gene transfection efficiency. Here, in order to develop new polymeric gene vectors with low cytotoxicity and high gene transfection efficiency, we synthesized a cationic polymer composed of low molecular weight polyethylenimine (PEI) of molecular weight of 600 Da cross-linked by 2-hydroxypropyl-γ-cyclodextrin (HP γ-CD) and then coupled to MC-10 oligopeptide containing a sequence of Met-Ala-Arg-Ala-Lys-Glu. The oligopeptide can target to HER2, the human epidermal growth factor receptor 2, which is often over expressed in many breast and ovary cancers. The new gene vector was expected to be able to target delivery of genes to HER2 positive cancer cells for gene therapy. The new gene vector was composed of chemically bonded HP γ-CD, PEI (600 Da), and MC-10 peptide at a molar ratio of 1:3.3:1.2. The gene vector could condense plasmid DNA at an N/P ratio of 6 or above. The particle size of HP γ-CD-PEI-P/DNA complexes at N/P ratios 40 was around 170-200 nm, with zeta potential of about 20 mV. The gene vector showed very low cytotoxicity, strong targeting specificity to HER2 receptor, and high efficiency of delivering DNA to target cells in vitro and in vivo with the reporter genes. The delivery of therapeutic IFN-α gene mediated by the new gene vector and the therapeutic efficiency were also studied in mice animal model. The animal study results showed that the new gene vector HP γ-CD-PEI-P significantly enhanced the anti-tumor effect on tumor-bearing nude mice as compared to PEI (25 kDa), HP γ-CD-PEI, and other controls, indicating that this new polymeric gene vector is a potential candidate for cancer gene therapy. © 2009 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/67144
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2009.11.012
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

77
checked on Dec 18, 2017

WEB OF SCIENCETM
Citations

74
checked on Nov 22, 2017

Page view(s)

36
checked on Dec 17, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.