Please use this identifier to cite or link to this item: https://doi.org/10.1364/BOE.3.000206
Title: Improving signal-to-noise ratio of structured light microscopy based on photon reassignment
Authors: Singh, V.R.
Choi, H.
Yew, E.Y.S.
Bhattacharya, D.
Yuan, L.
Sheppard, C.J.R. 
Rajapakse, J.C.
Barbastathis, G.
So, P.T.C.
Issue Date: 1-Jan-2012
Source: Singh, V.R.,Choi, H.,Yew, E.Y.S.,Bhattacharya, D.,Yuan, L.,Sheppard, C.J.R.,Rajapakse, J.C.,Barbastathis, G.,So, P.T.C. (2012-01-01). Improving signal-to-noise ratio of structured light microscopy based on photon reassignment. Biomedical Optics Express 3 (1) : 206-214. ScholarBank@NUS Repository. https://doi.org/10.1364/BOE.3.000206
Abstract: In this paper, we report a method for 3D visualization of a biological specimen utilizing a structured light wide-field microscopic imaging system. This method improves on existing structured light imaging modalities by reassigning fluorescence photons generated from off-focal plane excitation, improving in-focus signal strength. Utilizing a maximum likelihood approach, we identify the most likely fluorophore distribution in 3D that will produce the observed image stacks under structured and uniform illumination using an iterative maximization algorithm. Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods. © 2011 Optical Society of America.
Source Title: Biomedical Optics Express
URI: http://scholarbank.nus.edu.sg/handle/10635/67100
ISSN: 21567085
DOI: 10.1364/BOE.3.000206
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