Improvements in confocal microscopy imaging using serrated divided apertures
Gong, W. Si, K. Sheppard, C.J.R.
Si, K.
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Abstract
Confocal microscopy with serrated divided apertures is presented. Compared with using two smooth D-shaped apertures in confocal microscopy, the serrated divided apertures can suppress the out-of-focus central bright spot, thus increasing the efficiency of rejection of scattered light. Diffraction analysis shows that the serrated apertures maintain the optical sectioning strength while attenuating the background coming from far from the focal plane. In addition, the signal to background ratio (S/B) is also improved. © 2009 Elsevier B.V. All rights reserved.
Keywords
Angular gating, Confocal microscopy, Serrated divided apertures, Signal to background ratio
Source Title
Optics Communications
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Date
2009-10-01
DOI
10.1016/j.optcom.2009.06.044
Type
Article