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|Title:||Improved spatial resolution in fluorescence focal modulation microscopy|
|Authors:||Gong, W. |
|Citation:||Gong, W., Si, K., Chen, N., Sheppard, C.J.R. (2009). Improved spatial resolution in fluorescence focal modulation microscopy. Optics Letters 34 (22) : 3508-3510. ScholarBank@NUS Repository. https://doi.org/10.1364/OL.34.003508|
|Abstract:||We show that the focal modulation microscopy (FMM), which combines a spatial phase modulator with confocal microscopy, results in an improvement in spatial resolution. This technique was introduced to increase imaging depth into tissue and rejection of background from a thick scattering object. A theory for image formation in FMM is presented, and the effects of detecting the in-phase modulated fluorescence signal are discussed. Compared with conventional confocal microscopy, the width of the point-spread function for the in-phase fluorescence signal is improved by 16.4%. When applied to saturable fluorescence, the half-width at half-maximum is improved by 33.6%, 50.0%, and 62.9%, at demodulation frequencies 2ω, 4ω, and 8ω, respectively. © 2009 Optical Society of America.|
|Source Title:||Optics Letters|
|Appears in Collections:||Staff Publications|
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