Please use this identifier to cite or link to this item:
|Title:||Improved spatial resolution in fluorescence focal modulation microscopy|
|Authors:||Gong, W. |
|Citation:||Gong, W., Si, K., Chen, N., Sheppard, C.J.R. (2009). Improved spatial resolution in fluorescence focal modulation microscopy. Optics Letters 34 (22) : 3508-3510. ScholarBank@NUS Repository. https://doi.org/10.1364/OL.34.003508|
|Abstract:||We show that the focal modulation microscopy (FMM), which combines a spatial phase modulator with confocal microscopy, results in an improvement in spatial resolution. This technique was introduced to increase imaging depth into tissue and rejection of background from a thick scattering object. A theory for image formation in FMM is presented, and the effects of detecting the in-phase modulated fluorescence signal are discussed. Compared with conventional confocal microscopy, the width of the point-spread function for the in-phase fluorescence signal is improved by 16.4%. When applied to saturable fluorescence, the half-width at half-maximum is improved by 33.6%, 50.0%, and 62.9%, at demodulation frequencies 2ω, 4ω, and 8ω, respectively. © 2009 Optical Society of America.|
|Source Title:||Optics Letters|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 22, 2018
WEB OF SCIENCETM
checked on Jul 4, 2018
checked on Jul 6, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.