Please use this identifier to cite or link to this item:
|Title:||Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: In vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system|
|Citation:||Rath, S.N., Pryymachuk, G., Bleiziffer, O.A., Lam, C.X.F., Arkudas, A., Ho, S.T.B., Beier, J.P., Horch, R.E., Hutmacher, D.W., Kneser, U. (2011-05). Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: In vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system. Journal of Materials Science: Materials in Medicine 22 (5) : 1279-1291. ScholarBank@NUS Repository. https://doi.org/10.1007/s10856-011-4300-0|
|Abstract:||Smart matrices are required in bone tissueengineered grafts that provide an optimal environment for cells and retain osteo-inductive factors for sustained biological activity. We hypothesized that a slow-degrading heparin-incorporated hyaluronan (HA) hydrogel can preserve BMP-2; while an arterio-venous (A-V) loop can support axial vascularization to provide nutrition for a bioartificial bone graft. HA was evaluated for osteoblast growth and BMP-2 release. Porous PLDLLA-TCP-PCL scaffolds were produced by rapid prototyping technology and applied in vivo along with HA-hydrogel, loaded with either primary osteoblasts or BMP-2. A microsurgically created A-V loop was placed around the scaffold, encased in an isolation chamber in Lewis rats. HA-hydrogel supported growth of osteoblasts over 8 weeks and allowed sustained release of BMP-2 over 35 days. The A-V loop provided an angiogenic stimulus with the formation of vascularized tissue in the scaffolds. Bone-specific genes were detected by real time RT-PCR after 8 weeks. However, no significant amount of bone was observed histologically. The heterotopic isolation chamber in combination with absent biomechanical stimulation might explain the insufficient bone formation despite adequate expression of bone-related genes. Optimization of the interplay of osteogenic cells and osteo-inductive factors might eventually generate sufficient amounts of axially vascularized bone grafts for reconstructive surgery. © Springer Science+Business Media, LLC 2011.|
|Source Title:||Journal of Materials Science: Materials in Medicine|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 12, 2018
WEB OF SCIENCETM
checked on Dec 12, 2018
checked on Oct 27, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.