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Title: Enhanced background rejection in thick tissue using focal modulation microscopy with quadrant apertures
Authors: Si, K.
Gong, W. 
Chen, N. 
Sheppard, C.J.R. 
Keywords: Axial resolution
Confocal microscopy
Focal modulation microscopy
Imaging penetration depth
Quadrant apertures
Signal level
Transverse resolution
Issue Date: 1-Mar-2011
Source: Si, K., Gong, W., Chen, N., Sheppard, C.J.R. (2011-03-01). Enhanced background rejection in thick tissue using focal modulation microscopy with quadrant apertures. Optics Communications 284 (5) : 1475-1480. ScholarBank@NUS Repository.
Abstract: Image formation in focal modulation microscopy using quadrant apertures (QFMM) is presented. The spatial resolution is discussed: compared with confocal microscopy, QFMM can simultaneously enhance the axial and transverse resolution. In contrast to focal modulation microscopy using D-shaped apertures (DFMM), QFMM can maintain x-y symmetry of spatial resolution, thus causing less confusion in imaging interpretation. The capability of background rejection is investigated, showing QFMM has the potential to increase imaging penetration depth. The signal level is also analyzed, indicating that the preferred detector pinhole radius is greater than for DFMM, resulting in a higher signal level. © 2010 Elsevier B.V. All rights reserved.
Source Title: Optics Communications
ISSN: 00304018
DOI: 10.1016/j.optcom.2010.11.007
Appears in Collections:Staff Publications

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