Please use this identifier to cite or link to this item: https://doi.org/10.1097/BRS.0b013e31821986b3
Title: Effects of radial compression on a novel simulated intervertebral disc-like assembly using bone marrow-derived mesenchymal stem cell cell-sheets for annulus fibrosus regeneration
Authors: See, E.Y.-S. 
Toh, S.L. 
Goh, J.C.-H. 
Keywords: annulus fibrosus
bioreactor
bone marrowderived mesenchymal stem cell
cellsheet
tissue engineering
Issue Date: 1-Oct-2011
Source: See, E.Y.-S., Toh, S.L., Goh, J.C.-H. (2011-10-01). Effects of radial compression on a novel simulated intervertebral disc-like assembly using bone marrow-derived mesenchymal stem cell cell-sheets for annulus fibrosus regeneration. Spine 36 (21) : 1744-1751. ScholarBank@NUS Repository. https://doi.org/10.1097/BRS.0b013e31821986b3
Abstract: Study Design.: The aim of this study was to develop a tissue engineering approach in regenerating the annulus fibrosus (AF) as part of an overall strategy to produce a tissue-engineered intervertebral disc (IVD) replacement. Objective.: To determine whether a rehabilitative simulation regime on bone marrow-derived mesenchymal stem cell cell-sheet is able to aid the regeneration of the AF. Summary of Background Data.: No previous study has used bone marrow-derived mesenchymal stem cell cell-sheets simulated by a rehabilitative regime to regenerate the AF. Methods.: The approach was to use bone marrow-derived stem cells to form cell-sheets and incorporating them onto silk scaffolds to simulate the native lamellae of the AF. The in vitro experimental model used to study the efficacy of such a system was made up of the tissue engineering AF construct wrapped around a silicone disc to form a simulated IVD-like assembly. The assembly was cultured within a custom-designed bioreactor that provided a compressive mechanical stimulation onto the silicone disc. The silicone nucleus pulposus would bulge radially and compress the simulated AF to mimic the physiological conditions. The simulated IVD-like assembly was compressed using a rehabilitative regime that lasted for 4 weeks at 0.25 Hz, for 15 minutes each day. Results.: With the rehabilitative regime, the cell-sheets remained viable but showed a decrease in cell numbers and viability. Gene expression analysis showed significant upregulation of IVD-related genes and there was an increased ratio of collagen type II to collagen type I found within the extracellular matrix. Conclusion.: The results suggested that a rehabilitative regime caused extensive remodeling to take place within the simulated IVD-like assembly, producing extracellular matrix similar to that found in the inner AF. © 2011, Lippincott Williams & Wilkins.
Source Title: Spine
URI: http://scholarbank.nus.edu.sg/handle/10635/67019
ISSN: 03622436
DOI: 10.1097/BRS.0b013e31821986b3
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